- Free worldwide shipping, tracked and insured
- Same day dispatch, customs handled
- Certificate of analysis for every batch
- NNMT enzyme inhibition research
- Adipocyte lipogenesis and fat-mass models
- NAD+ and SAM metabolism studies
Overview
What is 5-Amino-1MQ?
5-Amino-1MQ is a water-soluble, membrane-permeable small molecule that inhibits nicotinamide N-methyltransferase (NNMT). It is used as a research compound in studies of adipocyte lipogenesis, NAD+ and S-adenosylmethionine metabolism, and muscle stem-cell ageing in cell culture and mouse models. Supplied for in-vitro and laboratory research use only.
Mechanism in research
How 5-Amino-1MQ is studied
In published research, 5-Amino-1MQ inhibited NNMT with a low-micromolar IC50 by binding the nicotinamide substrate site, and was selective against related methyltransferases and NAD+ salvage enzymes. In cultured 3T3-L1 adipocytes it reduced lipogenesis and altered 1-methylnicotinamide and NAD+ levels. In diet-induced obese mice, subcutaneous treatment for eleven days was associated with about 35% lower epididymal fat mass, more than 30% smaller adipocytes, and around 30% lower total cholesterol with no change in food intake, and a 28-day study reported dose-dependent limits on weight gain with better glucose tolerance and less hepatic steatosis. In aged mice, NNMT inhibition activated senescent muscle stem cells and improved regeneration after injury. These findings describe the published cell and mouse literature; there are no published human studies, and the compound is supplied here strictly as a research reference standard.
Reported targets & pathways
- Nicotinamide N-methyltransferase (NNMT)
- Nicotinamide, SAM, and 1-methylnicotinamide metabolism
- Adipocyte lipogenesis pathways
- Muscle stem-cell activation (aged mouse models)
Reference data
5-Amino-1MQ specifications
- Structure
- 5-amino-1-methylquinolinium iodide, C10H11IN2 (CAS 42464-96-0)
- Classification
- Small-molecule NNMT inhibitor (quinolinium salt), not a peptide
- Appearance
- Dry powder, hygroscopic
- Solubility
- Soluble in water and bacteriostatic water
- Molecular formula
- C10H11IN2
- Molecular weight
- 286.11 g/mol
- Purity
- 99.47%
- CAS number
- 42464-96-0
- PubChem CID
- 66522933
Research applications
Where 5-Amino-1MQ is used in research
Areas of study described in the published literature. Listed for research reference only.
NNMT inhibition assays
Used as a reference inhibitor in enzyme-kinetics and selectivity assays against related methyltransferases.
Adipocyte metabolism
Studied in cultured 3T3-L1 adipocytes for effects on lipogenesis and NAD+-related metabolite levels.
Diet-induced obesity models
Explored in high-fat-diet mouse models for changes in fat mass, glucose tolerance, and liver fat.
Muscle ageing research
Applied in aged-mouse studies of muscle stem-cell activation, regeneration, and exercise response.
Selected research
Published studies on 5-Amino-1MQ
Peer-reviewed references for further reading. Findings describe in-vitro and animal research; mechanisms and outcomes in humans are not established.
Biochemical Pharmacology · 2018
Reported that 5-Amino-1MQ reduced lipogenesis in cultured adipocytes and, in diet-induced obese mice treated subcutaneously for eleven days, was associated with about 35% lower epididymal fat mass, over 30% smaller adipocytes, and around 30% lower cholesterol with unchanged food intake.
Diabetes, Obesity and Metabolism · 2024
Reported a 28-day study in diet-induced obese mice where daily treatment limited weight and fat gain in a dose-dependent way, improved glucose tolerance and insulin sensitivity, and reduced hepatic steatosis, with distribution to adipose, muscle, and liver tissue.
Biochemical Pharmacology · 2019
Reported that in 24-month-old mice treated after muscle injury, NNMT inhibition increased muscle stem-cell activation and improved regeneration of the injured muscle compared with vehicle.
Journal of Medicinal Chemistry · 2017
Described the quinolinium series from which 5-Amino-1MQ was selected, reporting low-micromolar NNMT inhibition through binding at the nicotinamide substrate site.
Quality
Independently verified, batch by batch
Tested every batch
Every batch of 5-Amino-1MQ is independently analyzed by a third-party laboratory before it is released.
Independently verified
Identity and purity are confirmed by an independent third-party laboratory. This batch is verified to 99.47% purity.
Certificate of analysis
Every batch is independently tested with a certificate of analysis. A digital COA is available on request.
Reviews
What researchers say about 5-Amino-1MQ
tbh didnt expect a non peptide to dissolve that fast
ordered the 50mg for a muscle stem cell project in old mice, first non peptide ive bought from a peptide shop lol. went into water instantly, no swirling. halifax in 9 days with customs, vials all sealed. numbers from the first injury cohort are close to whats in the 2019 paper so the PI has stopped asking where i bought it
why does the courier only try at 7am?
The 5-Amino-1MQ itself is fine. We use it in a diet-induced obesity cohort and the glucose tolerance curves from the first group moved the way the 2024 paper describes, so the compound is not the problem. The courier is the reason this is four stars and not five. They attempted delivery at ten past seven two mornings running, before anyone had unlocked the building, then sent it back to a depot the far side of the ring road and I had to go and collect it in a taxi. Support at Miro were apologetic and offered to chase but by then the parcel was already sitting on a shelf with my name on it. Nine days in the end, most of them on that shelf.
l'inibitore di riferimento che ci mancava
lo usiamo come inibitore di riferimento negli LC-MS sul metabolismo del NAD+ e la 1-metilnicotinamide nelle cellule trattate scende come in letteratura, il rapporto NAD+ resta leggibile. prima lo prendevamo da un fornitore americano e ogni volta c'era da aspettare la dogana un mese perché arrivava da fuori UE, stavolta trieste in sei giorni. prezzo onesto per dieci flaconi, sopratutto rispetto a quello che pagavamo prima
Zoll hat nicht mal geschaut
Hab ehrlich gesagt damit gerechnet dass der Zoll bei einem Quinoliniumsalz zwischen lauter Peptiden nachfragt, aber das Paket war in fünf Tagen in Salzburg und keiner hat reingeschaut. Im HFD-Mausmodell sehen wir bisher das was die 2018er Arbeit beschreibt, Fettmasse runter bei gleicher Futteraufnahme. Bestellt haben wir das 10mg, das reicht für die Kohorten eine Weile.
Twice now
Second box of the 5mg for our 3T3-L1 work, lipid droplet staining dropped in treated cells same as with lot one, back to Málaga.
ic50 matches the SAR paper
we needed a reference inhibitor for nnmt kinetics and the only other route was a custom synthesis quote with a six week lead. ordered the 10mg here instead, bordeaux in eight days. ic50 in our assay came out low micromolar, which is where the 2017 SAR paper puts it, so its what it says on the vial. hygroscopic salt though, not a peptide, weigh it out quick and get the vial back in the desiccator. learned that the first afternoon
5-Amino-1MQ FAQ
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